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📌 This e-book documents my ongoing journey in bioinformatics engineering, integrating concepts from data science, software development, and biomedical research. Its aim is to systematically curate this learning process and develop a collection of searchable and reusable knowledge resources.

For a complete overview, see the 📚 Table of Contents.


📖 Topics Covered

  • Next-generation sequencing data analysis, including DNA-seq, RNA-seq, single-cell RNA-seq, and microbiome workflows

  • Data science methodologies, covering data preprocessing, analysis, and visualization

  • Statistical foundations and applied statistical analysis for biomedical and computational research

  • DevOps practices, including containerization, CI/CD, workflow automation, and reproducible research environments

  • Backend development, focusing on API design, server-side logic, and service architecture

  • Frontend development, with modern web technologies and frameworks

  • Cloud and networking concepts, including cloud infrastructure, container orchestration, and networking fundamentals

  • Core computer science topics, such as algorithms, data structures, and system-level concepts

  • Programming language syntax and usage, with practical examples across commonly used languages

  • Miscellaneous topics and supplementary notes


  • snakemake-rnaseq pipeline: A modular and reproducible RNA-seq analysis pipeline supporting Docker, Conda, and CI/CD—designed for both research and teaching applications.


📬 Contact

This e-book is actively maintained and will be updated on an ongoing basis. Comments, corrections, and scholarly or technical feedback are welcome.

🌐 Personal Website: Visit My Website 📩 Contact: benson106075@gmail.com 🔗 LinkedIn: Benson Lee 💻 GitHub: benson1231 🐳 Docker Hub: benson1231

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